Genomic analysis of a functional haloacid-degrading gene of Bacillus megaterium strain BHS1 isolated from Blue Lake (Mavi Gölü, Turkey)

Abstract Purpose Bacillus megaterium strain BHS1, isolated from an alkaline water sample taken Mavi Gölü (Blue Lake, Turkey), can grow on minimal medium containing 2,2-dichloropropionic acid. We characterized this bacterium at the genomic level. Methods The HiSeq platform was used to carry out genome sequencing, de novo assembly, and scaffolding with BHS1. Next, data were analyzed demarcate DNA regions protein-coding genes determine function of certain BHS1 genes. Finally, results a colorimetric chloride ion–release assay demonstrated that produces dehalogenase. Results De assembly sequence revealed size ~ 5.37 Mb average G+C content 38%. predicted nuclear harbors 5509 genes, 1353 tRNA 67 rRNA 6 non-coding (mRNA) Genomic mapping its amenability synthesize two families dehalogenases (Cof-type haloacid dehalogenase IIB family hydrolase type II), suggesting these enzymes participate in catabolism halogenated organic acids. identified seven Na + /H antiporter subunits are vital for adaptation environment. Apart pairwise analysis well-established L-2-haloacid dehalogenases, whole-cell strongly suggested II might act stereospecifically L-2-chloropropionic acid, D,L-2-chloropropionic Whole-cell studies confirmed utilization three substrates gene’s role dehalogenation. Conclusions To our knowledge, is first report full which enabled characterization selected having specific metabolic activities their roles biodegradation compounds.